Jonathan E Ploski

2 Questions 15 Answers 0 Followers

Questions related from Jonathan E Ploski

Does anyone know of rat and mouse cell lines that transduce well with AAV (any serotype)?

We get fantastic AAVDj transduction of 293 cells (human). However in comparison we find that mouse N2a, NIH 3t3, and Ht22, and Rat2 (rat) cell lines do not transduce well at all. Thank you in...

06 June 2015 9,433 0 View

Can someone recommend an adherent rat cell line that is easily transfected?

I like Human Hek293 cells because they transfect so well - usually ~95% with Lipofectamine. However I need to work with a Rat cell line to test CRISPR gRNAs in and having a rat cell line that...

04 April 2015 5,709 12 View