How to dye DOPC liposome with Dio c18 fluorescent dye?

13 November 2020 9,244 3 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View

I am using a rat TNFa ELISA to measure TNFa in culturing media. How soon following my alcohol treatment should I collect and measure TNFa?

I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...

16 September 2020 7,020 2 View

Can anyone help me with quality of seed roots in this picture?

So I grew some radish seeds in a petri-dish using two different biochars. Is it correct to conclude that the seed number 2 is healthier than number 1? Can anybody help me to understand the growth...

12 March 2020 6,645 5 View

Has anyone using organotypic hippocampal slice culture models observed holes in their tissue specimens between 6-12 days in vitro?

Our lab has noticed that after several tissue preparations for organotypic hippocampal slice cultures, some of our tissue is developing what appears to be large holes between the CA1 and dentate...

03 March 2020 3,981 3 View

After washing the activated carbon to remove its impurities, in an industrial scale, what are the ways of disposing the dirty liquid ?

A common practice in the production of activated carbon is post washing the material with water or acids to remove impurities. Now in an industrial scale that is a lot of wastewater to deal...

03 January 2020 2,212 7 View

How to improve fluid chart monitoring/recording?

I am currently doing a service improvement project at university, based on improvement of fluid balance charts, regarding monitoring and recording, more specifically for surgical high care...

20 September 2019 619 3 View

What are your parallel processing solutions? Is it possible to request time time on sophisticated computer equipment at your university?

I'm trying to run a tensorflow program that requires at least a cuda cpu (I think). Google Cloud has a 300 dollar voucher for their parallel processing service. From what I understand, the 300...

01 September 2019 7,218 3 View

What is the contact time(in industry) for powdered activated carbon in wastewater treatment?

I want to study the behavior of different powdered activated carbons (PAC) in removing of contaminants from wastewater. However, I am not sure what would be the ideal contact time for industries...

28 July 2019 1,101 10 View

Network Analysis Question: When is a small n too small?

I have been working on network analyses to assess DSM-IV PTSD symptomology using CAPS data for my study sample of women who have recently experienced interpersonal violence. I have created partial...

30 July 2018 2,482 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View