What parameters of quality control would I have to consider for antimicrobial toothpaste?

Hello, my name is Bianca and as a project for university I have assigned the manufacturing of antimicrobial toothpaste- quality control department. I tried to research online but I could not find...

17 January 2021 918 3 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

What criteria do you have in mind while characterizing a scientific theory?

I hesitate a lot before raising the question just mentioned. As RG is mainly a scientific forum, it is natural that the concept of theory appears in many posts from many posters. But what are...

25 October 2020 7,643 34 View

Would anyone have an explanation of the occurrence of this phenomenon in the LS-Dyna simulation in the drum seaming roll process?

Hello, I am simulating in Ls-Dyna a drum seaming process that consists of closing a drum, ie the lid with the body using two additional tools (figure 1). The tools are set as rigid shells and the...

26 August 2019 4,808 1 View

How to write a methodological paper?

Dear all, I would like to write a methodological paper. Notably, I would like to explain how a particular methodology can be used to study problems related to a specific field of studies (i.e....

21 October 2018 2,254 22 View

Has anyone dealt with sample crystallization during the purification steps of SPPS?

Hi all, We've been synthesizing some short (16 aa) peptides lately and they cleave beautifully from the resin, yielding a beautiful bulky white cloud in a 50:50 solution of hexanes/diethyl...

03 June 2018 631 2 View

Could you suggest must-read literature of socio-ecological systems?

Hi everyone, I might be approaching the field of socio-ecological systems to write the final paper of my PhD. I would like to get an idea of a super MUST-READ literature to get an overview of the...

04 April 2018 7,009 26 View

Are there any tools that can be used by students to analyse a brand's presence in social media, without having actual access to that brand's outlets?

some students of mine wish to analyse specific brands' social media presence; although a deep analysis is impossible if you're not part of that brand's team, maybe some tools could provide some...

18 December 2017 5,165 4 View

Has anyone one used (snap)frozen tissue samples in a ProcartaPlex Multiplex Immunoassay?

The guidelines states that it is suitable for serum, plasma and cell cultured lysates. Theoretically can you use animal tissue and make a tissue lysate?

21 September 2017 8,408 2 View

Regarding the use of water samples, what other testing methods can be used besides Coliform test for water that are more efficient and cost-friendly?

19 April 2017 5,018 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View