Does anybody know a book or a comprehensive meta-analysis about psychological aspects of nutritional behaviors, such as food choice or food intake?

Is there any book about the effect of psychological factors on food choice or amount of food intake?

02 May 2019 676 3 View

Methods of Thawing Frozen Mammalian Cells?

I am looking for different methods of mammalian stem cells freezing and thawing. Both in industrial and laboratory scale. Would greatly appreciate your input.

13 February 2019 5,485 10 View

Is it necessary for the sample size of an interventional study to be assigned equally into the two groups?

13 October 2016 4,762 4 View

Xrd vs DSC

To study crystallinity of materials, which method is better? When is it more accurate to use DSC or XrD?

24 May 2013 1,709 8 View

How can I disperse MMT nanoparticles within carrageenan- or acrylamide-based hydrogel?

Is there any surfactant needed? My MMT nanoparticles have hydrophilic behaviour.

28 January 2013 3,893 1 View

What is the best way to prepare a nanocomposite hydrogel with uniformly dispersed nanoparticles?

What are the factors to disperse nanoparticles (eg CNT, Fe2O3, Ag so on) within the hydrogel network?

07 November 2012 7,365 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View