Is a poly-glycine or poly-glycine/serine linker necessary with 3X FLAG tag?

28 February 2014 0 6K Report

I am planning to add 3X FLAG tag to a protein sequence and upon my literature search I found that there have been studies where 6 Glycine linkers were used between the protein and the FLAG tag. Is this absolutely necessary and a routine procedure or one can do without it?

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