Detecting brain cancer with convolutional neural network(CNN): How to choose hyperparameters (i.e.number of filters, filter shape, max-pooling shape)?

Hi everyone, I've decided to detect brain cancer form an image library, so I wanna use the CNN algorithm to extract features from the library and train my neural network but still I wanna know how...

19 August 2020 3,734 3 View

What is an appropriate time interval for correlation of annual SPEI time series?

Hi! I'm comparing two 50-year SPEI (Standardized Precipitation Evapotranspiration Index) time series created from model and observed data (1950-1999) and averaged over a large area. Both time...

14 October 2019 1,459 3 View

Is it possible to calculate the average pore width using DFT-model?

I have tried to calculate/estimate the average pore size using DFT-model asuming slit formed pores. I am using the ASAP 2020 instrument but the instrument only gives me the total volume of the...

30 June 2019 8,406 3 View

Are there known factors/parameters affecting the sialylation/desialylation of recombinant glycoproteins produced in cell cultures?

Can a human recombinant glycoprotein produced in mammalian cell culture lose part of its sialic acid residues due to changing conditions in the culture or the purification of the protein? Any...

17 May 2019 9,954 3 View

How do I interpret a summary ROC curve with maj. of pts in up right?

Hi I am reviewing a figure for a senior member of my group and getting confused on the sROC or summary ROC curve. It is a meta analysis data set The sROC curve points are nearly all up in the...

03 April 2018 2,901 0 View

Why can't I detect p-Akt bands in my western blots?

I am looking for phospho-Akt (serine 473) in rat heart tissue samples using western blots. I have done trouble shooting for weeks but still nothing. At first we thought it was our antibodies that...

06 June 2016 4,922 5 View

What is the best tissue homogenization method for reverse lectin ELISA?

Our initial plan is to use streptavidin or neutravidin coated 96-well plates (15115, Thermo Scientific) and biotinylated lectins to isolate glycoproteins from complex samples then probe for...

10 June 2015 7,426 2 View

How much tissue of a mouse heart is sufficient for protein extraction?

Does anyone know how much tissue is needed of a mouse heart to obtain sufficient protein for western blotting? We would like to cut a mouse heart longitudinally and use one half for blots and the...

24 March 2015 7,681 7 View

Can anyone recommend a method for Mining Gene Expression Omnibus (GEO) for the expression of 1 gene?

17 October 2014 3,008 5 View

Does anyone have experience in TNF-alpha detection: ELISA vs Western Blots?

Good day For my MSc I wanted to determine the levels of TNF-a released by H9c2 cells in response to treatment with Doxorubicin. I treated every day for 5 days, so after each day I collected the...

06 August 2014 2,818 18 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How to perform CV by using potentiostat?

I have deposited a metal oxide thin film of glass plate, A conductive thin film was also deposited on the glass substrate before the deposition of metal oxide film. How can I perform its CV from a...

01 February 2021 7,710 3 View

How to implement truss element with no effect of compression in ansys workbench static structural?

I was trying to add ligaments in cervical spine geometry. There it is mentioned in the existing literature to add the ligaments in the geometry as truss element with no effect of compression....

03 January 2021 2,205 0 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

What is the Standard Operating Procedure(SOP) for anthocyanin extraction from red rice?

Anthocyanins are rich in antioxidant properties. Previous research has shown that red rice contains a good amount of anthocyanins. While there are a number of laboratory procedures for anthocyanin...

24 August 2020 5,159 7 View

How to tell or calculate group difference in subgroup meta-analysis?

Dear all, For some time, we have been working with Stata metaprop in order to analyse our results from our review on two surgical techniques (for now A and B). Through the metaprop command...

03 August 2020 4,693 1 View

Enriching Operating Room (OR) Based Student Learning: How Should We Structure it? What models are appropriate for medical students?

Various models like Apprenticeship model, BID model, Koen's Model, One Minute Preceptor (OMP), 4C/ID model etc have been previously utilized for surgical resident's training. Dynamics of student...

29 July 2020 3,308 3 View

How to determine the sample size with Two independent variables with continuous outcome in which no clinical studies have been performed?

I am writing a synopsis for my dissertation on a topic in which I'm comparing 2 endodontic sealers clinically (RCT). there are no clinical studies for their comparison in literature till now. I am...

25 July 2020 8,846 3 View

Assessment of T-cells in the surgically removed Skin tissue ?

How can we assess the presence and amount of T-cells in the freshly removed skin tissue after surgery or punch biopsy? Also, if we freeze the skin tissue at -20C for a long time, after thawing to...

10 July 2020 5,208 4 View