Toni Leigh Goldswain

6 Questions 10 Answers 0 Followers

Questions related from Toni Leigh Goldswain

Is 100ug/kg ghrelin, administered to rats, clinically or physiologically relevant?

Many of the papers I have read use this concentration (twice daily), but I have not come across how they chose this concentration. Is anybody able to help with this?

06 July 2017 706 0 View

Why can't I detect p-Akt bands in my western blots?

I am looking for phospho-Akt (serine 473) in rat heart tissue samples using western blots. I have done trouble shooting for weeks but still nothing. At first we thought it was our antibodies that...

07 June 2016 5,022 5 View

Is there a 'rule of thumb' for deciding whether to measure markers in serum versus plasma?

I have seen it done both ways in the literature and I am unsure of which (serum or plasma) to use. I would like to measure TNF-alpha, cardiac troponin T and brain natriuretic peptide. Thank you!

28 September 2015 2,590 4 View

Which is the best route for Doxorubicin administration? Intravenous or intraperitoneal?

My application for ethics was deferred because the reviewers said that administering Doxorubucin via an i.p injection will cause blistering and that it should be given i.v, however, every paper...

08 June 2015 2,757 6 View

How much tissue of a mouse heart is sufficient for protein extraction?

Does anyone know how much tissue is needed of a mouse heart to obtain sufficient protein for western blotting? We would like to cut a mouse heart longitudinally and use one half for blots and the...

25 March 2015 7,778 7 View

Does anyone have experience in TNF-alpha detection: ELISA vs Western Blots?

Good day For my MSc I wanted to determine the levels of TNF-a released by H9c2 cells in response to treatment with Doxorubicin. I treated every day for 5 days, so after each day I collected the...

07 August 2014 2,908 18 View