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Questions related from Toni Leigh Goldswain
Many of the papers I have read use this concentration (twice daily), but I have not come across how they chose this concentration. Is anybody able to help with this?
06 July 2017 749 0 View
I am looking for phospho-Akt (serine 473) in rat heart tissue samples using western blots. I have done trouble shooting for weeks but still nothing. At first we thought it was our antibodies that...
07 June 2016 5,094 5 View
I have seen it done both ways in the literature and I am unsure of which (serum or plasma) to use. I would like to measure TNF-alpha, cardiac troponin T and brain natriuretic peptide. Thank you!
28 September 2015 2,649 4 View
My application for ethics was deferred because the reviewers said that administering Doxorubucin via an i.p injection will cause blistering and that it should be given i.v, however, every paper...
08 June 2015 2,806 6 View
Does anyone know how much tissue is needed of a mouse heart to obtain sufficient protein for western blotting? We would like to cut a mouse heart longitudinally and use one half for blots and the...
25 March 2015 7,830 7 View
Good day I was told a few weeks back that GAPDH cannot be used as a loading control in differentiated cells since its expression does not stay constant. Has anybody heard anything about this?
25 August 2014 5,784 8 View
Good day For my MSc I wanted to determine the levels of TNF-a released by H9c2 cells in response to treatment with Doxorubicin. I treated every day for 5 days, so after each day I collected the...
07 August 2014 2,968 18 View
Hi everyone, I am treating differentiated C2C12s with Doxorubicin. I probed for phospho and total Akt. Unlike T-Erk and T-Stat which remained fairly constant, the T-Akt is changing and I would...
15 July 2014 8,565 2 View
