Which Phase of cell cycle is most radiation sensitive.
Chinese hamster cells in M and early S phases are most radiosensitive, and those in late S phase are most radioresitant. For more details, please see the following paper
https://www.ncbi.nlm.nih.gov/pubmed/5924188
Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.
07 August 2017 2,293 5 View
Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?
07 August 2017 9,508 1 View
Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”
07 August 2017 4,712 7 View
Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?
07 August 2017 945 5 View
An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...
07 August 2017 8,415 7 View
I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...
07 August 2017 5,462 4 View
What are some common treatments that could be used to disrupt the antibody-antigen complexes?
07 August 2017 5,565 4 View
What is the function of the enzyme bound to the secondary antibody?
07 August 2017 8,198 9 View
What is the need for serial dilution of the sample when performing an Elisa?
07 August 2017 1,692 1 View
What are the differences and similarities between western blotting and immunohistology?
07 August 2017 2,178 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View