Intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

More Paulina Żelechowska's questions See All

How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View

Problem with YES estrogen bioassay. Estrogen contamination?

Hello everyone, I've been encountering a recurring problem with the YES (Yeast Estrogen Screen) test, specifically related to the development of an intense red color after adding CPRG. I'm using...

07 February 2024 1,144 0 View

Is there a way to separate plasma from lyzed red blood cells when whole blood samples have been frozen at -80°c?

I am currently engaged in the analysis of a collection of previously obtained samples, which primarily comprise frozen whole blood. My specific requirement pertains to isolating the plasma...

08 January 2024 1,842 1 View

Anyone have any fairly recent info/research on human and non-human primate bonding?

At the moment I am interested in human and non human primate bonding, especially in captive management, but more widly too. The causes and consequences, pros and cons, how it could inform our...

17 December 2023 9,236 0 View

Problem with Trizol RNA extraction - what should be purity of chloroform and isopropanol?

Hi, I'm trying to isolate RNA from cell cultures using the Trizol method. However, all my samples have low 260/280 values (~1.6). Could this be because I used chloroform and isopropanol, which was...

26 October 2023 9,909 6 View

How can I know the number of particles per vial of the pH rhodo s aureus bioparticle reagent?

Hello, I am working with the aforementioned reagent, but my problem arises that the reagent does not indicate the quantity of particles that come per vial, that is, the number of particles, it...

11 October 2023 817 1 View

Can someone recommend a good CRISPR identifier?

Hi, I am a beginner in bioinformatics and I would like to identify CRISPRs in my MAGs fasta files. Can someone recommend an up-to-date good tool that can be easily installed through the Conda...

21 May 2023 3,549 3 View

How calculate heat gain in PVT modules?

how to calculate heat gain from solar cells in pvt modules

27 April 2023 6,289 1 View

Do warnings of non-convergence in a trait.glm analysis always indicate that the model is not valid?

I'm working with a trait.glm analysis from the mvabund package in R. I have two datasets that look exactly the same with the same amount of environmental variables and the same functional traits,...

15 February 2023 6,547 3 View

Does anyone know how to run an latent moderated mediation in mplus?

The problem is that model indirect is not available for type is random option. Thanks

14 February 2023 1,269 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View