Dear Colleague
I am starting a project on miRNA expression in serum/plasma of patients with breast cancer, regarding to exiqon, miR-93-5p, miR-103a-3p, miR-423-3p, miR-423-5p, miR-191-5p and miR-425-5p are suitable for such sample, if you have similar experience, please let me know which of them are better to start ?
thanks in advance
Dear Dr. Tahmasebi
5s rRNA is the gene that can be considered as internal control as we used for the profiling of circulating miRNAs in plasma of breast cancer patients. Also, U6 can employed.
With Best Regards
Esmaeil Babaei
Dear Dr.Babaei
Thanks, but if you check the exiqon or literatures , you will see that U6 and 5s are not recomended specially for liquid samples
Dear Maryam,
If there is any published paper on this topic, I suggest you to check which internal gene, other researchers have used for the same sample (breast cancer, plasma/serum).
Best wishes,
PN
As we experienced, 5s rRNA is actually detected in plasma of breast cancer patients and related paper is under submission. Anyway, I agree with Dr. Nikpour comment.
EB
hello dear,
I would also suggest to check the expression stability of whatever miRNA or snRNA you used as endogenous controls between samples. sometimes geometric mean of two endogenous controls may give better results. i like U48 alot any sample i used, specially u48 worked very well but dont know about its change in plasma of cancer patients. Good luck
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