Hi all,
I am planning to start some experiments in the IntelliCage system using DREADD injected mice. When testing DREADD injected mice, the CNO i.p. injection is required (please note, my other experiments are done using i.p. injection, I would keep this condition consistent). And the DREADD effect may last for up to 6 hours. Question 1, What is the best timing for injecting the CNO? I am afraid some of the mice may start the tasks when the DREADD effect is gone. Question 2, How to avoid the repeated injection of CNO-caused pathological changes, rather than only the cell manipulation?
Any suggestions would be greatly appreciated
W
hello,
About the question1, I am not sure which parameter is mainly focused, so I can only give a general idea. Based on my results, the effect of CNO was observed from 30-120 min, so I recommended to perform the test 1hr after injection (for short trial such as 10-30min). If you want to know the time in the day to do the injection...I need more information, otherwise, I can`t answer it.
About the Q2, I could not find out which paper indicated this result. If you can give more information, it will be more helpful.
Hope this is helpful.
Hi Wulaer,
This paper gives some time course of effects of CNO dihydrochloride on freely moving mice (ip injection) which might be of help: Article cAMP-producing chemogenetic activation of indirect pathway s...