Hi Chiara, this is a really basic question, but have you stained your gel just to make sure that you have a band with the expected size where Integrin alpha 3 should be?

Yes, I agree Ricardo 's opinion, this is a really basic question. There are always ahout trouble -shooting in Santa Cruz when you purchased such antibodes. Please ask the dept. of Technical Support. Good Luck.

Dear Ricardo, dear Ellen,

thanks for your answers.

Do you mean to check with ponceau?

Yes, or coomassie blue, just to make sure that you actually have protein there...

try the millipore one. It works.

Ehy everyone!

I would like to inform you that, after a lot of tries (including ponceau, comassie, antibodies, buffers, SDS gel conditions, lysis buffers, cooking time, ecc..) I could finally find an antibody wich worked in WB!! It's again from Santa Cruz clone I-19.

For human keratinocytes in culture gives a weak but detectable band around 130 kDa. Then... yes, it was hard.. O_O

Thank you very much for your suggestions and support *

Hi, Chiara

I have the same trouble with integrin alpha 3, but in human monocytes. I will test the Santa Cruz clone I-19. I would like know the conditions for WB, as BSA or milk blocking, reducing or non-reducing condition and dilution.

Could you help me, please?