I recently purchased "Recombinant mouse Leukemia inhibitory factor" from Isokine (# 01-A1140 ). How can I convert 10 μg of packaging in units/ml?
I still don't know How can I convert 10 μg of packaging in units/ml? but now i know according to the data sheet 10 μg = 1000000 unit/ml
Hi Vahideh,
I should have said: already answering. Sorry.
The data sheet says: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
And that 10 micrg is equivalent to 10e6 units.
Then, resuspending in 100 microliters of sterile water containing 0.1%BSA will give you a concentration of 100mcrg/ml which is equivalent to 10e7units/ml.
There you go!! LIF ready to use!!!
Hope this helps.
Best,
Josema
Hi Vahideh,
if you resuspend LIF as I indicated to obtain a solution of 10e7 units/ml, then using the formula that you told me c x v = c' x v' you will know how much of the LIF solution you should add to any volume of media that you want for obtaining a final concentration of 1000 unit/ml, which is the concentration that is usually used to maintain the undifferentiated state of mES.
Good luck,
Josema
- Badges
- Science method