I usually use chick embryo (9-11 days old) for inoculation of influenza in allantoic route. Has anyone used 13 days old chick embryo for allantoic inoculation of influenza and worked with him?
Thank you Shamus for your answer. Actually, I am using these eggs for virus titration, so I don't care for the volume. I will only run spot HA on allantoic fluids from these eggs. Embryo death during inoculation would be very significant in the results in that case.