Industrial cultivation of macroalgae

More Elham Alinezhad Sardareh's questions See All

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why do I get no melt curve peak and no Ct for some of my patients but not others, in a SYBR green qPCR?

I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...

31 May 2024 1,088 0 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Anyone with phosphate flotation experience?

I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...

14 May 2024 4,505 4 View

Why my amino acids do not contribute in amidation reaction?

Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...

15 April 2024 6,778 3 View

How to increase concentration and quality of RNA after extraction?

Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...

11 March 2024 9,171 8 View

How to interpret my samples' results from D1000 ScreenTape Assay?

Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...

10 March 2024 8,764 0 View

Can anyone help with SHSY5Y cells differntiation?

Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...

19 February 2024 1,672 5 View

Why Cryo cells (freezed cells) do not attached to the flask?

Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...

19 February 2024 9,305 2 View

Can someone suggest protocol to produce knock-down cells by using shRNA lentivirus particles?

Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...

10 January 2024 6,425 0 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

What did the plantation economy in the South produced and all the crops cultivated in north India cannot cultivated in south India why?

26 June 2024 7,264 0 View

What are the best ways to cultivate self-discipline?

I would like to become self disciplined and work by myself without supervision. How do I achieve it when I am not interested at all in working without any push by others?

24 June 2024 4,529 5 View

Can we culture human epidermal keratinocytes in DMEM culture media?

Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...

24 June 2024 2,609 3 View

Wheather HYVs are alowed in Natural farming ?

One of the major objectives of Natural Farming is self-reliant farmer and promotion of traditional varieties so that the farmers can use their own seeds. But it is observed that most of the...

11 June 2024 5,696 0 View

Does dry weather limit tillage options for timing cultivation to complement conservation plans?

02 June 2024 1,735 5 View

What specific challenges do heat waves pose to vegetable cultivation in India?

28 May 2024 5,155 1 View

How to accelerate the growth of dermatophytes in culture medium?

Hello. I have cultivated the species of dermatophytes in the special culture medium of SCC. Unfortunately, the rate of species growth is very slow. Does anyone have any experience in this field,...

26 May 2024 4,552 3 View

Can someone tell me what is this on my petri dish?

Hi! I left some petri dishes with Pseudomonas aeruginosa cultivated in MHI in the fridge (8°C) during some time off, and when I came back, it was like this. This is just one plate out of many, and...

24 May 2024 5,149 3 View

Hello, any advices on how to cultivate insulinoma NIT-1 cells in vitro?

For a set of experiments, we need to use insulinoma NIT-1 cells (ATCC CRL-2055). First time we get them from ATCC, none of the cell attached to the flask. We just received a second vial of cells,...

21 May 2024 9,390 0 View

Clive James Fox

My institute conducts quite a lot of research into macroalgae cultivation including overseas - you might want to look up Prof Liz Cook at Scottish Association for Marine Science.

Geddada Mohan Narasimha Rao

We have conducted some outdoor commercial cultivation of Kappaphycus and Gracilaria at visakhapatnam, East coast of India. I am pleased to collaborate for discussion and exchange of technical knowledge.

Elham Alinezhad Sardareh

Hi dear Dr.

I hope you are well. How can I have a meeting to exchange information?

Whatsapp number:00989304871800

[email protected]

Geddada Mohan Narasimha Rao Clive James Fox I hope you are well. How can I have a meeting to exchange information?

Dear Dr Elham.

Sorry for my late response... initially give your questions through email i will respond then if you satisfied we will talk through a zoom call