6 Questions 11 Answers 0 Followers
Questions related from Elina Garcia Rodriguez
Hello everyone, I ask for your guidance regarding these unknown black dots that seem to be in my cell flask. The cells are macrophages derived from human blood, and the culture is around 2 weeks...
01 January 2020 4,338 4 View
Hello all, I am having some serious trouble staining resin embedded rat bone using basic fuchsine and toluidine blue. I have tried many different protocols but it seems like the bone matrix does...
01 January 2020 1,866 6 View
Hello! I am having trouble understanding what a modified paragon staining contains? I know that normal paragon staining contains toluidine blue and basic fuchsine but have not found any...
11 November 2019 2,449 5 View
Hello all! I have som trouble staining beta galactose in my MC3T3 cells. Here is my protocol: - Cells stressed with H202 treatment. - washed twice in PBS - fixated in 4% paraformaldehyde 5min...
07 July 2019 3,297 5 View
Hello! I want to stress osteoprogenitor cells and thereafter measure it, either in the cells or in the media. Whats the best approach for this? The best approach i have right now is to use H202...
06 June 2019 5,387 3 View
I am gonna optimise an incubation buffer for proximity extension assay (PEA), and I am wondering if I am missing anything vital in the buffer. This buffer is mainly for cell lysate detecting...
11 November 2018 8,108 1 View
