Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Where can I find these two papers?

14 January 2021 1,572 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Contamination when culturing primary uterine endometrial cells?

Dear everyone I'm working on the endometrium of the uterus. I need to extract endometrial cells from the endometrial tissue. Usually, I will separate the tissue into 2,3 mm pieces and incubated in...

25 November 2020 7,428 3 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Why case-controlled study is more bias prone than cohort study?

I noticed from several articles saying that case-controlled study is more bias prone than cohort study (in the context of cancer early detection using molecular marker). I spent sometime to try to...

17 November 2020 1,878 4 View

Does PROCESS v3.5 by Andrew F. Hayes with SPSS Statistics 27 on MacOS 10.15.7 work?

After installing the PROCESS v3.5 tool, I ran a mediation model 4 with three variables (N = 125). The warnings below showed up on the output window, and the status at the bottom shows, "Running...

15 November 2020 3,930 14 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View