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Questions related from Cheng-Maw Ho
We used to use NADPH as the gene used for nornalization in rat liver experiment. However, NADHP ( and beta actin as well?) may be altered in the setting of fatty liver related experiment. We use...
26 November 2023 1,971 1 View
Dear Colleague, I use PKH26 for cell tracking after hepatocyte transplantation. We found that if we look directly for red flurescence with DAPI staining, the labeled cells can be found much more...
08 January 2023 3,285 3 View
We are trying to identify X and Y chromosome in tissue and probe the presence of X chromosome in human tissue by using PCR for specific DNA (for example, ATL1 for X chromosome; SRY for Y...
17 February 2022 2,377 3 View
When we perform housekeeping genes (such as GADPH) for baseline comparison control in RT-PCR analysis, the degrees of CT values were found to vary a lot between different animals (in our case, 5...
20 October 2021 4,223 2 View
I want to perform a GEE analysis with repeated-measured factors and survival analysis (like Cox regression but with repeated measured variables) in SPSS. How do I proceed with this dataset? I had...
29 August 2020 1,244 3 View
I am checking CD3+ cells in liver using fluorescent immuno-histochemistry. I found some cells are CD3+ but not express CD4, CD8, CD25, and CD56. What are these cells anyway?
22 August 2019 5,956 4 View
I used two M2 marker (CD206 and CD163) to identify the M2 macrophage but they had quite different expression patterns in histology. Are there different expression spectrums between M2 markers?
28 February 2016 7,124 3 View
What is the morhology of Canal of Hering in liver tissue? Can I use CK 19 only to identify them (in small cuboidal cells) around portal area?
22 October 2015 435 4 View
What do you think of this research topics? I launched a special section on this in Frontiers in Surgery. The link is...
01 January 1970 1,491 0 View