Hi, I did some scRNAseq as well as protein expression analysis on the same sample, however, the RNA level is not consistent with the protein level. How do people usually explain this piece of data in manuscripts? Thanks!
It has been well-established that there is a low correlation between RNA expression level and protein level. Here is a review article on the subject.
https://www.cell.com/cell/pdf/S0092-8674(16)30270-7.pdf
Hello Kun Yun
mRNA levels cannot be used as surrogates for corresponding protein levels without verification.
Please refer to the paper attached below.
https://kendricklabs.com/wp-content/uploads/2016/08/WP1_mRNAvsProtein_KendrickLabs.pdf
You may also want to refer to a few more articles attached below.
Article The utility of protein and mRNA correlation
Article Maier T, Güell M, Serrano L.. Correlation of mRNA and protei...
Best.
Hi
Usually, mRNA level does not have correlatation with its protein level. There are many factores that can interfer in this correlation. Usually, protein concentrations correlate with the corresponding mRNA levels by only 20 – 40%.
see: doi.org/10.1186/1471-2164-10-365
doi.org/10.1186/1471-2164-9-246
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