Might be a rookie question however the abcam platelet isolation protocol stipulates re-suspension of washed platelet pellet in Tyrode's with 3mg/mL BSA (fresh added).

I have been isolating platelets and re-suspending them for multiple assays without the inclusion of BSA.

Just seeing from other's perspective is BSA inclusion is absolutely necessary and if so why is it important?

Thank you for your time :)

http://docs.abcam.com/pdf/protocols/isolation-of-human-platelets-from-whole-blood.pdf