36 Questions 69 Answers 0 Followers
Questions related from Mac Dz
Need to check quorum sensing activity of Vibrio campbellii.
21 January 2021 787 5 View
Out of 50 mg/ml stock solution of plant extract, 20 μl is taken which is equivalent to 1000 μg/ml and transfer to 5 ml of broth (first tube). Other tubes were filled with 4 ml of broth and 1 ml is...
18 August 2020 3,895 27 View
GIIs= ZDI in combination / total of ZDIs of the two agents in single action in order to corroborate the synergistic activity of the antibiotic in combination with the plant extracts, and synergy...
24 May 2020 9,268 5 View
3 hrs, 12 hrs or 24 hrs? Agar pre-diffusion will be conducted at 4 °C
28 April 2020 8,931 26 View
Step 1. Wash & Freeze dry the plant material. Step 2. 20g of plant powder mix with 100ml of solvent & macerated for 72 hours under room temperature (28℃). Step 3. Use rotary evaporator...
26 April 2020 10,066 35 View
The anti-serum will consist of antibodies extracted from those who already recovered from the infection.
23 March 2020 5,572 11 View
How to prepare 50% DMSO from 100% DMSO? What is the solution should I use to dilute it? I have water and chloroform. The plant material is initially extracted in chloroform. Need to...
17 December 2019 5,845 8 View
What would be the concentration of a 10μl aliquot taken from a 10ml solution with a concentration of 1000mg/ml?
31 August 2019 889 9 View
What are the chances of increasing IELTS band score for writing & speaking by 0.5 after a remark (EOR)?
28 May 2019 4,370 11 View
Which plant extract is better for antimicrobial sensitivity testing (disk diffusion assay)? centrifuged or non-centrifuged extract?
24 February 2019 2,261 8 View
Any risk of contamination? In case of antibiotic sensitivity testing?
03 February 2018 5,739 27 View
Maceration of plant material in distilled water at room temperature (30°C) for 24 hours? or Maceration of plant material in distilled water at 4°C for 24 hours?
15 January 2018 2,075 8 View
What is the difference between the plant species Bunchosia armeniaca and Bunchosia glandulifera? Please do post pictures if you have.
07 January 2018 3,119 10 View
Multiple publication of findings related to bioactivities of the same plant species?
13 December 2017 3,140 8 View
25°C? 28°C? 30°C? 35°C? Which one is optimum?
20 November 2017 9,138 16 View
Is it necessary to lower the pH of Mueller-Hinton agar supplemented with 2% glucose in order to prevent bacterial contamination? Can we add conc HCL to lower the pH?
15 November 2017 8,730 20 View
Generally, which solvent gives best results for a plant extract (larger zone of inhibition) against E. coli? Ethanol or Methanol?
26 September 2017 9,716 21 View
Lets say 95% Ethanol or 95% Acetone?
17 September 2017 8,792 13 View
Select the answer out of A,B,C or D
27 August 2017 3,500 6 View
Agar pre-diffusion at room temperature or at 4°C?
07 August 2017 4,453 11 View
Would 1ml of 0.5 McFarland inoculum be too much for mixing with 25ml of MH agar in a 100x15mm petridish? I'm planing to use pour-plate technique for antimicrobial susceptibility testing with agar...
24 July 2017 2,047 13 View
Any other recommendable methods? Is it alright just to leave the alcohol immersed plant extract to open air with the containers lid open for the evaporation?
26 February 2017 8,531 3 View
Which is a better antibiotic to take as a control? Ciprofloxacin or Gentamicin in plant extract antimicrobial sensitivity testing against E.coli?
11 November 2016 9,471 3 View
How long will it take to evaporate 100ml of 95% ethanol in the refridgerator at 4℃?
06 November 2016 8,238 2 View
In case of pour plate method for agar well diffusion assay..
17 October 2016 5,530 16 View
freezer temperatures at -20 & -80? or is it best to store at refrigeration temperatures at 0-4 °C does cold temperatures have any harmful effect on phytochemicals of a plant extract containing...
13 October 2016 1,349 10 View
Does autoclaving changes the pore size of these membrane filters
10 October 2016 6,290 2 View
Can Streptococcus pyogenes and viridans be grown & cultured on Brain Heart Infusion (BHI) Agar for antibiotic sensitivity testing?
23 September 2016 3,669 5 View
Any temperature conditions? Can bacteria grow in saline solution?
22 September 2016 3,182 6 View
Ethanol Methanol Isopropyl alcohol Please state the best one & rank them
08 September 2016 4,105 16 View
Dose filtering a plant extract with 0.45 µm syringe filter reduce the concentration of phytochemicals? Dose UV irradiation changes the chemical structure of phytochemicals in a plant compound?
03 September 2016 7,172 3 View
is it more than 1 ml or less? if less than 1 ml then how can we calculate µg/ml?
26 August 2016 8,961 5 View
Why do glass petri dishes crack from the bottom when I hit them with the bunsen flame (for further sterilization)?
25 August 2016 1,466 8 View
- Please state any reference values - How to calculate LDL cholesterol in albino wistar rats?
19 August 2016 8,766 7 View
Do we have to seal the lower part of the well with agar or just leave it unsealed
26 July 2016 5,915 4 View
When mentioning scientific names like Sedum caespitosum (Cav.) DC. can we just mention Sedum caespitosum without (Cav.) DC? to make the name short?
23 May 2016 267 7 View
