In the picture below we tried to isolate plasmid by Alkali Lysis but in the circled region we couldn't visualize anything .What may be the reasons?

18 June 2023 1 573 Report

We tried to isolate plasmid by Alkali Lysis method. In the circled region we could not visualize so what may be probable reasons for that

Paul Rutland

Possibly when you washed the precipitated plasmid the dna pellet was not sufficiently well centrifuged and it was lost in aspiration or tube inversion

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