Jnr-diff
At position 732 my forward and reverse readings are incompatible. what could be the reason for this. there is no problem in the others. there are two different peaks at this position in the...
05 February 2024 3,994 0 View
There is a jump in the expected Ct change when I go from dilution 10^6 to 10^5. When I prepare my standard curve, the most diluted standards have a difference of around 3 cycles, that would be...
05 September 2023 4,675 0 View
Hello, When preadipocyte cells were seeded to differentiate and confluent 3 days later, detachment and peeling occurred in all wells when changing the culture medium. 60,000 for 12 wells. I am...
23 July 2023 5,967 3 View
I want to reuse the cellulose membranes I used in the experiments. Can you help me find a procedure on this?
23 April 2023 9,936 0 View
The solution I prepared is not stabilizing. The depositing process is happening instantaneous. After a couple of seconds, free nickels in solution just precipitates. Any idea about preventing this...
25 November 2022 9,007 0 View
I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you,
01 January 2022 7,880 3 View
I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you, Ezgi
01 January 2022 598 1 View
Edit: image uploaded I've blotted for beta-actin on cell culture protein lysates many times but the beta-actin detection range was not between 37 - 50 kDa range but 25 - 37 kDa range each time....
09 September 2021 4,074 4 View
I have a sample with 138 participants. Only 6 of them reported living alone (%4.3) while the remaining 132 share the household with others (family/partner/housemate etc.) I am trying to decide...
23 July 2021 3,872 6 View
For example if I use a throttling valve in order to cool a gas by using the joule thomson effect, how can I adjust the valve so that the gas reduces its pressure to a desired value? For example...
11 July 2021 8,140 4 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
What could be the reason for mixotrophs to be yellow too instead of green? Thank you
08 July 2024 594 2 View
I'm currently analyzing the Raman spectra of coloured plastic samples and have encountered a wavy, oscillating pattern in the spectra of some coloured samples. I was wondering what the possible...
04 July 2024 1,949 4 View
Which river basin has the lowest total replenishable groundwater resources and utilization of irrigation potential created lags behind the potential created what are the reasons?
03 July 2024 9,638 0 View
