In the isolation of E.coli O157:H7 from en-reached stool sample, which media would likely be used step-wisely for its isolation? What if EMB is used?

15 February 2018 1 4K Report

I am interested only on E. coli and used selective media Eosin methylene blue agar media. is there any probability of missing the isolate if those colony with metallic sheen will be transfered for further identification?

Dawit Kassaye

A bit there is difficulty since some bacterial isolates can also demonstrate a metallic sheen colony on Eosin methylene blue agar medium. hence it needs the use of antimicrobial supplement to avoid such concerns.

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Science method

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