Does anybody know of a method to measure, in situ, amplification of short DNA segments (< 50 bp) obtained through PCR without having to do electrophoresis? Preferred methods would be based on absorption, CD, or fluorescence, in that order.
Protoporphyrin (IX) ferrochelatase has an absorption peak in the UVC. Aleksandar Simeonov and I have written a paper suggesting that the fundamental molecules of life are really pigments absorbing...
05 June 2014 7,147 1 View
Because we have to raise our solutions to high temperature we sometimes have evaporation and therefore sticking of the organic molecules, DNA, polymerase, etc. to the inside surfaces of the quartz...
07 August 2013 1,965 13 View
Popper called Darwin's theory of evolution through natural selection a "metaphysical research program" principally because of the apparent tautology in "survival of the survivors". However, beyond...
07 August 2013 4,053 63 View
Thermodynamics seems to have an uncanny relation to general relativity. For example, the zeroth, first, second and third laws of thermodynamics have their analogs in the physics of black holes...
06 July 2013 4,583 70 View
Life, for the most part, dissipates short-wavelength photons. Hurricanes dissipate long-wavelength photons. What are some other differences between these two types of non-linear, non-equilibrium...
05 June 2013 4,993 21 View
All our experiments with different DNA in standard buffer give a broad extinction peak at 515nm. Does anyone know where this comes from? We suspect that it is light scattering, but we can't find...
05 June 2013 7,591 0 View
Can anyone tell me what is the shortest length of primer that is necessary for extension by polymerase for DNA?
04 May 2013 1,241 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View