All our experiments with different DNA in standard buffer give a broad extinction peak at 515nm. Does anyone know where this comes from? We suspect that it is light scattering, but we can't find reports of it in the literature.
Protoporphyrin (IX) ferrochelatase has an absorption peak in the UVC. Aleksandar Simeonov and I have written a paper suggesting that the fundamental molecules of life are really pigments absorbing...
05 June 2014 7,147 1 View
Because we have to raise our solutions to high temperature we sometimes have evaporation and therefore sticking of the organic molecules, DNA, polymerase, etc. to the inside surfaces of the quartz...
07 August 2013 1,965 13 View
Popper called Darwin's theory of evolution through natural selection a "metaphysical research program" principally because of the apparent tautology in "survival of the survivors". However, beyond...
07 August 2013 4,053 63 View
Thermodynamics seems to have an uncanny relation to general relativity. For example, the zeroth, first, second and third laws of thermodynamics have their analogs in the physics of black holes...
06 July 2013 4,583 70 View
Life, for the most part, dissipates short-wavelength photons. Hurricanes dissipate long-wavelength photons. What are some other differences between these two types of non-linear, non-equilibrium...
05 June 2013 4,993 21 View
Can anyone tell me what is the shortest length of primer that is necessary for extension by polymerase for DNA?
04 May 2013 1,241 3 View
Does anybody know of a method to measure, in situ, amplification of short DNA segments (< 50 bp) obtained through PCR without having to do electrophoresis? Preferred methods would be based on...
31 December 2012 6,362 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View