What changes when activity is studies at a specific substrate concentration?

What does this statement mean - The uptake of substrate at a specific concentration may reflect both Km or Vmax or both. If Km of a transporter is independent of one of its regulator then the...

09 October 2013 479 7 View

Uncompetitive and non-competitive inhibition of enzyme

If an inhibitor is uncompetitive, i.e inhibits by binding to ES complex, thus decreasing the Km and Vmax value and if another inhibitor is non- competitive, i.e it inhibits by decreasing the...

09 October 2013 5,273 1 View

Vmax and Km effect on transporter?

If one regulator changes Vmax of a transporter and the other regulator works by changing Km, and when both regulators are effectively added, they do not show any additive regulatory effect on the...

08 September 2013 546 21 View

Glucose transport - can anyone help?

Considering the fact that the amount of glucose in the intestine is more than 5mM (i.e more than the normal blood plasma concentration), why would there still be an active transport of glucose by...

08 September 2013 7,475 9 View

Does anyone have experience with hIgh affinity yet decreased Vmax?

To understand an effect of protein kinase on substrate transport, an inhibition in transport was seen. Analyzing kinetics, the kinase caused decrease in Km of transporter. Indicating that the...

06 July 2013 7,894 4 View

How can I know what are the possible post translational modification for a particular protein?

If affinity of a protein changes, it might alter the post translational modification or might add a modification to the protein. How do I find which are the possible changes that occur to affect...

11 December 2012 4,091 7 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View