Normally, you would want your Delta's to be as high as possible. This indicates a big gap between the top match and the second match.
But what if it is zero? Does it then mean that there is only a single match? Is that good or bad?
If it is zero, there should be not second hit. Perhaps your database is rather small. Try larger databases, they will usually give score of 0.1 and more - which should be aimed for.
Best,
Christoph
Units to indicate the enzyme activity of thrombin seem to deviate from the "standard" units. How do I convert the NIH - units to micromoles / minute?
07 August 2016 1,717 2 View
This is what I did: I serially diluted my protein of interest in human serum. Concentrations were measured with an immunoassay. The protein was then captured using immunoprecipitation. Eluates...
10 November 2014 7,352 3 View
Before you all shout: "BLAST!"; that is of course the first thing that I tried. However, what I want is a list of proteins that contain exactly the peptide sequence that I query. BLAST does a...
09 October 2014 6,227 25 View
We are doing mass spectrometry on (semi-)purified protein samples. The aim is to reach a maximum amount of sequence coverage. Since we use a purified mixture, software like percolator eliminates...
05 June 2014 4,833 2 View
I want to calculate the area under the curve of the extracted ion chromatograms of my mass spectrometry dataset using Proteome Discoverer (Thermo Fisher). However, the area shows as zero for all...
03 April 2014 1,139 6 View
We have a working immunoprecipitation protocol for my protein of interest, which has recoveries over 90% when using small volumes of serum (200-500 uL). We now want to scale up and...
10 November 2013 1,954 6 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View