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Questions related from Alexander Streng
Units to indicate the enzyme activity of thrombin seem to deviate from the "standard" units. How do I convert the NIH - units to micromoles / minute?
08 August 2016 1,657 2 View
Normally, you would want your Delta's to be as high as possible. This indicates a big gap between the top match and the second match. But what if it is zero? Does it then mean that there is only a...
08 August 2015 5,899 1 View
This is what I did: I serially diluted my protein of interest in human serum. Concentrations were measured with an immunoassay. The protein was then captured using immunoprecipitation. Eluates...
11 November 2014 7,315 3 View
Before you all shout: "BLAST!"; that is of course the first thing that I tried. However, what I want is a list of proteins that contain exactly the peptide sequence that I query. BLAST does a...
10 October 2014 6,066 25 View
We are doing mass spectrometry on (semi-)purified protein samples. The aim is to reach a maximum amount of sequence coverage. Since we use a purified mixture, software like percolator eliminates...
06 June 2014 4,795 2 View
I want to calculate the area under the curve of the extracted ion chromatograms of my mass spectrometry dataset using Proteome Discoverer (Thermo Fisher). However, the area shows as zero for all...
04 April 2014 1,094 6 View
We have a working immunoprecipitation protocol for my protein of interest, which has recoveries over 90% when using small volumes of serum (200-500 uL). We now want to scale up and...
11 November 2013 1,905 6 View
