In processing fish samples for GC MS and/or heavy metal analysis, is there any difference between granded oven dried samples and ashed samples?

What is the difference between granded oven dried fish samples and ashed fish samples in processing fish samples for chemical analysis?

04 February 2021 9,942 3 View

Help with multivariate test selection?

Hi all, I have a dataset containing 2 independent variables (1. Region [North, East, South and West] and 2. Year [2010, 2015, and 2020]) and 4 continuous dependent variables (e.g. height, etc.)....

26 January 2021 9,329 3 View

How to plot data, if in a pair of values (for a paired t-test / Wilcoxon test) one of the two values is missing?

I have a dataset with 100 paired values (pre- and post intervention X). In 20 couples 1 of the 2 values is missing. When I compare pre- to post-intervention values, those 2 couples / rows will be...

04 December 2020 3,044 9 View

Python ODE: how to get the solution of last timestep with solve_ivp?

Hello, to solve a system of ODEs, I set up a Python-code with solve_ivp as ODE-solver. The solver is calling a function, which generates my ODE-system. Inside my ODE-system, I need the previous...

07 May 2020 914 2 View

Is there not a common root of LBM and SPH modelling incompressible fluid flows?

Is there not a common root of Lattice Boltzmann Methods (LBM) and Smoothed-particle Hydrodynamics (SPH) modelling incompressible fluid flows? I see common grounds by means of a mesocopic...

30 December 2019 3,550 7 View

How to interpret cross-level interactions in multilevel models?

My thesis-partner and I examine context level and individual level predictors effect on populist right-wing voting (i.e., Danish People's Party in Denmark) applying a multilevel logistic...

11 December 2019 7,738 2 View

AIM for the mouse?

I am looking for markers for Actrivation-induced markers in the mouse. In the human, for CD4: it's CD25, CD107a and OX40, and for CD8, it's PDL-1 (Bowyer et al., Vaccine 2018). In the mouse, CD44...

30 January 2019 9,592 2 View

How to run qualimap (command-line tool)?

I tried to run qualimap: http://qualimap.bioinfo.cipf.es/. When using the command ./qualimap, I am always getting the following error message: ./qualimap Java memory size is set to...

23 September 2018 9,438 3 View

Is the measured area in an image with a bigger scale in Y than in X dependent of the orientation?

I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...

06 June 2018 9,891 5 View

Which DNA isolation Kit for NGS (MiSeq, WGS of bacteria)?

Hello, for routine work, we are using the QIAamp DNA Mini Kit to isolate the DNA from bacteria. This kit works fine when the intention is to use the DNA for routine PCR. However, the yield is...

23 January 2018 8,282 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View