In nanoparticles preparation, not totally evaporated organic solvent, will cause drug to go out in supernatant during centrifugation?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Trying to make optimization for Mixer on different pdk ?

i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...

09 June 2024 9,729 3 View

Is it good choice to use the SARC/J auxiliary basis set with def2-SVPD basis set without including any relativistic effects like ZORA or whatever?

I’m currently utilizing the ORCA software for my computations, applying the PBE0 functional combined with the def2-SVPD basis set and incorporating the RIJCOSX approximation. For the auxiliary...

07 June 2024 6,352 0 View

Do the methods of calculating the absorption coefficient (Alpha) from absorbance differ between powder samples and thin films?how is it calculated ?

When using the absorption spectrum to determine the absorption coefficient (Alpha), do the calculation methods differ between powder samples and thin films? If there are differences, what are the...

07 June 2024 8,797 2 View

How do quick initial-test to select antibiotic-producing fungal strains?

We have dozens of pure fungal strains recovered from the soil and need to know the extent of their ability to produce antibiotics. Still, the problem is the spread of fungal mycelia on the plate...

09 May 2024 9,467 2 View

Does anyone have detailed methods and instructions on how to count the numbers of intraepidermal nerve fibers (IENFs)/mm using Fiji - ImgaeJ?

Counting the numbers of intraepidermal nerve fibers

05 May 2024 3,296 1 View

Effects of Aeromonas caviae and Aeromonas veronii on Freshwater Fish?

28 April 2024 9,191 1 View

Convert solid organic powder to nanoparticle?

i synthesized schiff base with bulk size., any suitable method to convert it in nanosize with full regards

22 April 2024 1,423 2 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View