In flow cytometry, how can separate Synechococcus-PC rich from Prochlorococcus using FACSCALIBUR cytometer?

Already acquired my samples and notice that Synechococcus-PC rich is overlapping with Prochlorococcus on the chart FL4, red vs FL2, orange. What should I do to discriminate each population? Or...

11 September 2015 10,049 2 View