Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

Key elements to consider before rolling out the final survey?

hi, for my quantitative research, i am analysing the pilot survey data. While, my cronbach is looking good, but Tstat, few outer loadings & Pval doesnt look good. Which are the key...

01 May 2020 6,426 10 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

I want to carry out analytical method development method for gadobutrol drug a MRI contrast reagent . Not able to get uv in these .could anyone help??

I not able to carry out uv response on these could any one suggest how to take uv as these compound dont have any chromophoric group present in these .

09 February 2020 3,291 3 View

Low library yield using Illumina True seq small RNA library prep ?

I am looking for suggestions to improve the yield of my libraries prepared using Illumina True seq small RNA library prep kit. Input RNA is purified small RNA (50 ng) and I run for 15 PCR cycles....

03 February 2020 2,645 2 View

Unit of power in line spectrum from conversion from log scale(dB) to linear scale(?) ?

I have a doubt about the unit of amplitude on y axis on linear plot of a sound wave generated in Matlab. In logarithmic scale it is dB but I am confused about what it should be on the linear scale...

04 September 2019 6,644 4 View

Why my single stranded RNA bands don't appear on a denaturing agarose gel?

I am trying to check the size of my single stranded RNA on agarose gel with a denaturing loading dye in TAE buffer. Although ssRNA ladder bands are appearing but my samples (having 25-100 base RNA...

10 April 2019 4,091 3 View

Which turbulence model give best results to capture flow characteristics in S shaped diffuser duct in context of ansys fluent?

computational fluid dynamics problem

03 September 2018 1,128 4 View

I am currently working on miBCI using BCIcompetition2 dataset iii. I used ica for processing. What could be further implied from the result obtained?

I am working on miBCI using the dataset above mentioned in python. For preprocessing of the same I've used a butterworth filter the result of which is then used for Independent Component...

12 June 2018 1,879 3 View

Which solvent system(s) can specifically be used to elute triterpenoids from leaf extracts using column chromatography?

I am using Chl:meth system to elute the triterpenoids through column chromatography using silica 60-120 mesh. But high amount of impurities are coming with the desired molecule. If the molecule...

25 April 2018 7,287 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View