well let me explain it in a short way.... In general after determining the optimal concentration for natural product-loaded nanogels, the next step is to apply them to treat cell lines.

This process involves a few critical steps, including cell line selection and culturing. It is important to select cell lines that closely mimic the intended pathological condition and culture them under optimal conditions to achieve the desired confluency.

The cultured cells should then be treated with the nanogels at the established optimal concentration. The duration of exposure should be aligned with the stability of the nanogels and the release kinetics of the natural product to ensure effective delivery.

After treatment, assess the cells using assays such as MTT for viability, LDH for cytotoxicity, flow cytometry for apoptosis, and confocal microscopy for intracellular uptake. These evaluations provide insights into the therapeutic efficacy and safety of the nanogels.

Data interpretation and further studies are necessary. Statistically analyse the collected data to draw meaningful conclusions. Comparative studies with other drug delivery systems or the free drug can highlight the benefits of the nanogels. Mechanistic studies on the action of the natural product within the cell lines can deepen understanding of its therapeutic potential.