Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

I want to carry out analytical method development method for gadobutrol drug a MRI contrast reagent . Not able to get uv in these .could anyone help??

I not able to carry out uv response on these could any one suggest how to take uv as these compound dont have any chromophoric group present in these .

09 February 2020 3,291 3 View

Low library yield using Illumina True seq small RNA library prep ?

I am looking for suggestions to improve the yield of my libraries prepared using Illumina True seq small RNA library prep kit. Input RNA is purified small RNA (50 ng) and I run for 15 PCR cycles....

03 February 2020 2,645 2 View

Why my single stranded RNA bands don't appear on a denaturing agarose gel?

I am trying to check the size of my single stranded RNA on agarose gel with a denaturing loading dye in TAE buffer. Although ssRNA ladder bands are appearing but my samples (having 25-100 base RNA...

10 April 2019 4,091 3 View

How to model Hasin 3D damage for composite in Abaqus?

Simply we can model hasin damage in Abaqus but that is 2D, gives only the inplane stresses not the perpendicular stress.I know VUMAT subroutine will be required for that, if anyone have some idea...

18 January 2019 7,711 8 View

I am currently working on miBCI using BCIcompetition2 dataset iii. I used ica for processing. What could be further implied from the result obtained?

I am working on miBCI using the dataset above mentioned in python. For preprocessing of the same I've used a butterworth filter the result of which is then used for Independent Component...

12 June 2018 1,879 3 View

Can anyone provide me the list of all Abaqus python functions used for calling events in Abaqus cae?

16 March 2016 2,429 2 View

Can someone help me with Real time PCR of genes at acidic pH?

Hi All, i am working real time PCR of certain genes at acidic pH. i have done the same experiment 4-5 times one year back and i got upregulation of certain genes. This work is also published by...

31 January 2016 3,721 4 View

Which is optimum cDNA concentration for RT PCR run?

Please suggest the optimum concentration of cDNA for RT PCR run. Does diluting the cDNA 1:10 or 1:100 help in increasing amplification, specially for low expressing target genes?

06 January 2016 6,148 4 View

Why can I get no ct value for my RT PCR experiment?

I am trying to amplify cytokine genes in cDNA derived from macrophages but unable to get any amplification using comparative delta Ct experiment. Cross checked mRNA concentration and purity which...

05 January 2016 9,725 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View