Impact of TCEP concentration on expression in BL21

Kin Wt @Kin-Wt

01 January 1970 0 4K Report

Hi! I heard that maybe it will be better to increase TCEP concentration to obtain more protein. What do you think?

Badges
Science topic

More Kin Wt's questions See All

Hi! Do you know why we use MgCl2 in lysis buffer?

Sometimes we can find in protocols recommendation to add magnesium chloride to lysis buffer. Why?

13 February 2024 8,400 1 View

How long should it take to grow a bacterial culture with 1:500 dilution ratio to early log phase?

Hello, I was making DH5a competent cells but I was confused with the condition of bacterial culture in the protocol I followed. The second step of the protocol is to "Dilute 20 μl of overnight...

04 September 2021 5,572 2 View

What are the critical merit function operands for F-theta lens system optimization on Zemax?

I ran into some issues optimizing for an f-theta lens group on Zemax with some attempts in flattening the field curvature and achieving the field size and work distance that are desirable. Does...

27 April 2020 7,392 0 View

What is the importance of examine personal micro-climate control of occupant during the thermal comfort assessment?

I have found some thermal comfort assessments included the survey of respondents' micro-climate control. May I know what is the importance of these questions and how does it link with the result...

17 January 2019 3,785 2 View

How to control the current supply from solar panel?

Hi, I am currently working on a photovoltaic system. May I know is there any device that is suitable to control the current supply to my load. Let say I am testing my load with 2 A, 3 A and 4 A,...

03 June 2018 4,790 8 View

Why does the light chain of the antibody shows a thicker band than the heavy chain on the blotting membrane?

We were doing the monoclonal antibody generation. After the western blotting incubation with IRDye and infrared fluorescence detection. Two bands were found on the sample column of the membrane....

01 December 2016 3,187 2 View

Patch primary human coronary artery smooth muscle cell (HCASMC) - whole cell configuration (electrophysiology)

Hello all, I am trying to patch primary HCASMC, it was working fine but all of the sudden everything upside down. I am wondering if anyone have any tricks / experience on patching HCASMC. Here is...

22 October 2015 4,182 8 View

Which are the top three most significant factors influencing the execution of effective last-mile structure in today's omni-channel retail operations?

Last-mile structure is the distribution structure enabling the last stretch of a business-to-consumer (B2C) parcel delivery. It takes place from the order penetration point (i.e., fulfilment...

17 October 2015 2,586 3 View

How common is a perfect diagnostic measure (100% Sens, Spec, PPV, NPV, AUC)?

I've performed analyses on a series of variables, 3 of which yielded 100% sensitivity, 100% specificity, 100% positive predictive value, 100% negative predictive value, AUC of 1.0 (1.0,1.0). I did...

27 August 2015 7,428 6 View

1.3 Protamine: 1 Heparin ratio has been shown to be effective for full reversal. What are the units of each?

Most studies just report the ratio but they don't report the units. Is 1.3:1 a ratio of 1.3 ug/mL protamine to 1 U/mL heparin (that doesn't seem to work out)?

09 April 2015 651 0 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,514 0 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

Do you think in which age of geology laterites can be more than other ages in Iran?

I want to know more about laterites in Iran.

31 July 2024 8,219 2 View

With attention to past my experiences on field geology I think any mineral ores can be in any geology area. Are you agree with me?

I want to know more about ore deposits in world.

27 July 2024 3,827 4 View

Why semicircle of nyquist plot is not complete?

While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...

26 July 2024 7,542 4 View