How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to get structure and topology for glycosaminoglycan (GAG) of arbitrary length?

Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...

23 December 2020 6,092 2 View

How can I synthesis heparin nano particle?

I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...

16 November 2020 9,455 2 View

Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

Causes of poor cell viability of WBCs after RBC lysis with 1X FACs Lyse buffer?

We are performing a whole blood lysis procedure on heparinized blood (3mL) to lyse RBCs and later remain with WBCs that we count and freeze down and later cryopreserved in liquid nitrogen for...

20 October 2020 7,677 6 View

I need a suggestion on the best RNA extraction kit that can be used for RNA extraction from blood sample collected in heparin?

I need a suggestion on the best RNA extraction kit that can be used for RNA extraction from blood sample collected in heparin

30 June 2020 9,867 4 View

How can i get heparin induced coagulopathy mice? One dose IV. or multidose SC. ?

Is just one intravenous dose of heparin enough to prolong bleeding and clotting time in mice?

23 March 2020 3,688 3 View

Trouble extracting DNA from mouse blood. suggestions?

Hi there, I am using Qiagen blood/tissue DNeasy kit on some heparinized fresh mouse blood (about 120ul) and I am getting only

02 March 2020 2,660 4 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

27 January 2020 496 1 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

31 December 2019 1,792 1 View

Does anybody have an optimized protocol for isolating RNA from human plasma samples with Trizol-LS?

31 December 2019 5,534 5 View