14 February 2024 1 8K Report

Sometimes we can find in protocols recommendation to add magnesium chloride to lysis buffer. Why?

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How long should it take to grow a bacterial culture with 1:500 dilution ratio to early log phase?

Hello, I was making DH5a competent cells but I was confused with the condition of bacterial culture in the protocol I followed. The second step of the protocol is to "Dilute 20 μl of overnight...

04 September 2021 5,572 2 View

What are the critical merit function operands for F-theta lens system optimization on Zemax?

I ran into some issues optimizing for an f-theta lens group on Zemax with some attempts in flattening the field curvature and achieving the field size and work distance that are desirable. Does...

27 April 2020 7,392 0 View

What is the importance of examine personal micro-climate control of occupant during the thermal comfort assessment?

I have found some thermal comfort assessments included the survey of respondents' micro-climate control. May I know what is the importance of these questions and how does it link with the result...

17 January 2019 3,785 2 View

How to control the current supply from solar panel?

Hi, I am currently working on a photovoltaic system. May I know is there any device that is suitable to control the current supply to my load. Let say I am testing my load with 2 A, 3 A and 4 A,...

03 June 2018 4,790 8 View

Why does the light chain of the antibody shows a thicker band than the heavy chain on the blotting membrane?

We were doing the monoclonal antibody generation. After the western blotting incubation with IRDye and infrared fluorescence detection. Two bands were found on the sample column of the membrane....

01 December 2016 3,187 2 View

Patch primary human coronary artery smooth muscle cell (HCASMC) - whole cell configuration (electrophysiology)

Hello all, I am trying to patch primary HCASMC, it was working fine but all of the sudden everything upside down. I am wondering if anyone have any tricks / experience on patching HCASMC. Here is...

22 October 2015 4,182 8 View

Which are the top three most significant factors influencing the execution of effective last-mile structure in today's omni-channel retail operations?

Last-mile structure is the distribution structure enabling the last stretch of a business-to-consumer (B2C) parcel delivery. It takes place from the order penetration point (i.e., fulfilment...

17 October 2015 2,586 3 View

How common is a perfect diagnostic measure (100% Sens, Spec, PPV, NPV, AUC)?

I've performed analyses on a series of variables, 3 of which yielded 100% sensitivity, 100% specificity, 100% positive predictive value, 100% negative predictive value, AUC of 1.0 (1.0,1.0). I did...

27 August 2015 7,428 6 View

1.3 Protamine: 1 Heparin ratio has been shown to be effective for full reversal. What are the units of each?

Most studies just report the ratio but they don't report the units. Is 1.3:1 a ratio of 1.3 ug/mL protamine to 1 U/mL heparin (that doesn't seem to work out)? 

09 April 2015 651 0 View

How to use double skin facades to improve microclimate?

Which type of building double skin facades will provide a good microclimate?

22 March 2014 4,735 2 View

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