Hi! Do you know why we use MgCl2 in lysis buffer?

Kin Wt @Kin-Wt

14 February 2024 1 8K Report

Sometimes we can find in protocols recommendation to add magnesium chloride to lysis buffer. Why?

Adam B Shapiro

DNase requires Mg2+ to function. If the lysis buffer contains DNase, that is the reason for the MgCl2.

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