Kin Wt

5 Questions 1 Answers 0 Followers

Questions related from Kin Wt

How can I isolate tRNA from E.coli?

Hello! Do you know any method for tRNA isolation using E.coli? I know that there are a lot of commercial kits but I prefer do it using reagents in lab. What is your experience?

24 March 2025 1,977 1 View

When and why should we use upflow on AKTA pure system?

I know that it is recommended during fixing column but I am curious if there are much more cases. Maybe it has a lot of applications ;)

04 June 2024 2,681 1 View

Hi! Do you know why we use MgCl2 in lysis buffer?

Sometimes we can find in protocols recommendation to add magnesium chloride to lysis buffer. Why?

14 February 2024 8,429 1 View

Impact of TCEP concentration on expression in BL21

Hi! I heard that maybe it will be better to increase TCEP concentration to obtain more protein. What do you think?

01 January 1970 3,856 0 View

Is it possible to see oligomers on SDS-PAGE?

Hello, I am concerning,if it is possible to see oligomers on SDS-PAGE gel. Theoretically, mass should be higher on gel but on the other hand during addiing buffer and boiling the samples all the...

01 January 1970 7,651 0 View