01 January 1970 0 2K Report

Currently, We are using free floating section in 12-well plates and small baskets to transfer tissues from one well to another. I have a few specific queries:

  • Could you please provide insights on the process you follow to clean the wells and baskets upon completing the procedure?
  • In terms of the baskets, do you maintain a consistent set throughout the entire procedure, or do you opt to replace them post-primary antibody incubation to prevent any antibody attachment to the basket mesh or walls?
  • Is it common practice for you to reuse plates for subsequent experiments?
  • I've been encountering challenges with the DAB staining phase. During the initial 5 minutes of DAB (with Ni) incubation, the sections appear to take on a light brown hue, which progressively transitions to a bluish tone and finally settles into a dark blue shade after around 15 minutes.

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