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Questions related from Priyadarshini Dutta
Currently, We are using free floating section in 12-well plates and small baskets to transfer tissues from one well to another. I have a few specific queries: Could you please provide insights on...
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I am working on Immunohistochemistry for Per1 protein expression on mouse brain coronal sections (40microns thickness, free floating). In the protocol that I'm following, it says that the DAB...
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I am currently working on IHC for Per1 protein expression in the suprachiasmatic nucleus of mouse brain coronal sections 40um thickness. The ABC kit that I have, has the expiry date of June 2022....
30 June 2023 3,260 5 View
I am working on Immunohistochemistry on mouse brain and have sectioned 40 microns thick sections as per the protocol. I will be using 12-well plates. How many sections are advisable to be kept per...
13 June 2023 9,580 6 View
I want to stain some of my mouse brain sections with Cresyl Violet. The mouse brains (not perfused, no PFA/Formalin used) were fresh frozen in OCT and cut in the cryostat at 15μm thickness. The...
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Characterizing a GPCR-ligand interaction is critical to understanding the biology of the receptor. As GDP/GTP exchange is one of the earliest events that follows ligand binding, monitoring GTP...
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