Priyadarshini Dutta

5 Questions 5 Answers 0 Followers

Questions related from Priyadarshini Dutta

For Immunohistochemistry, how do you decide the DAB exposure time?

I am working on Immunohistochemistry for Per1 protein expression on mouse brain coronal sections (40microns thickness, free floating). In the protocol that I'm following, it says that the DAB...

30 June 2023 2,620 4 View

ABC Vector Kit Expiration for Immunohistochemistry (IHC) ?

I am currently working on IHC for Per1 protein expression in the suprachiasmatic nucleus of mouse brain coronal sections 40um thickness. The ABC kit that I have, has the expiry date of June 2022....

30 June 2023 3,252 5 View

How many whole Mouse Brain Sections (40microns) can be used per well in a 12-well plate for IHC?

I am working on Immunohistochemistry on mouse brain and have sectioned 40 microns thick sections as per the protocol. I will be using 12-well plates. How many sections are advisable to be kept per...

13 June 2023 9,577 6 View

Cresyl Violet Staining - Fresh Frozen Mouse Brain (animal not perfused, brain not fixed in formalin, brain frozen in OCT)?

I want to stain some of my mouse brain sections with Cresyl Violet. The mouse brains (not perfused, no PFA/Formalin used) were fresh frozen in OCT and cut in the cryostat at 15μm thickness. The...

17 May 2023 7,606 4 View

Immunohistochemistry - Mouse Brain

Currently, We are using free floating section in 12-well plates and small baskets to transfer tissues from one well to another. I have a few specific queries: Could you please provide insights on...

01 January 1970 2,285 0 View