Hello, I need to quantify fluorescence in some confocal microscope pictures. Particularly, I would like to quantify the green channel fluorescence that is inside the nuclei of my cells, stained with DAPI. I'm trying to use Cell Profiler, but it's the first time I use this software so I ask you for suggestions. I would appreciate a lot a pipeline example for achieving my purpose. I attach one of my picture ( I separated the three channels). Thank you in advance.

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