Chiara Rossi

4 Questions 1 Answers 0 Followers

Questions related from Chiara Rossi

Lipid doplets quantification. Cell Profiler or ImageJ methods?

Hello everyone, could someone suggest me a method for the quantification of Lipid droplets Nile Red stained? I attach here some representative pictures. I would appreciate a lot if you can provide...

30 October 2018 7,472 1 View

It is good to use Alexa488 and Alexa555 in a colocalization experiment?

Hello, I used the secondary fluorescent antibodies AlexaFluor488 and AlexaFluor555 together in my immunofluorescence experiments. Now I would like to evaluate the colocalization of the two marked...

20 December 2017 5,810 3 View

Point s`read function in Coloc2. How to determine it?

Hello, I am using Coloc2 in ImageJ to quantifiy the colocalization between two fluorescent channels in my confocal images. The problem is that I should be able to indicate the value of the Point...

13 December 2017 7,977 1 View

How to measure colocalization in CellProfiler?

Good morning, could anyone suggest me a pipeline to run in Cell Profiler? I need to quantify colocalization between two proteins Here, I attach pictures of the two channels, red and green(SOD2 and...

07 March 2017 5,633 3 View