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Questions related from Chiara Rossi
Hello everyone, could someone suggest me a method for the quantification of Lipid droplets Nile Red stained? I attach here some representative pictures. I would appreciate a lot if you can provide...
30 October 2018 7,491 1 View
Hello, I used the secondary fluorescent antibodies AlexaFluor488 and AlexaFluor555 together in my immunofluorescence experiments. Now I would like to evaluate the colocalization of the two marked...
20 December 2017 5,818 3 View
Hello, I have a question about quantitative fluorescent microscopy. Maybe is an obvious one, but I would like to be sure about that. I am using fluorescent images to realize quantitative...
15 December 2017 1,789 2 View
Hello, I am using Coloc2 in ImageJ to quantifiy the colocalization between two fluorescent channels in my confocal images. The problem is that I should be able to indicate the value of the Point...
13 December 2017 7,996 1 View
Hello, I have a question about a mistake I made during my immunofluorescence protocol. I usually wash my coverslip in Triton X-100 0.1% in PBS before incubation with the secundary antibodies. The...
07 April 2017 9,521 6 View
Good morning, could anyone suggest me a pipeline to run in Cell Profiler? I need to quantify colocalization between two proteins Here, I attach pictures of the two channels, red and green(SOD2 and...
07 March 2017 5,660 3 View
Hello, I need to quantify fluorescence in some confocal microscope pictures. Particularly, I would like to quantify the green channel fluorescence that is inside the nuclei of my cells, stained...
24 January 2017 9,976 1 View
