Hello.

I want to ask about the way to make ROI from other image.

Please imagine streets and buildings.

I have a multi-fluorescent microscopic image and have a figure for each color (red, green, blue...).

I want to ignore some cells, like 'buildings'.

I have a image showing interstitium, like 'streets'.

I want to create ROI, which is correspond to 'streets', from this image.

By doing so, I think it is possible to ignore the information of cells.

I tried 'Analyze particle' but it did not work well (maybe inner cells is included in the ROI).

Could you tell me the way to create ROI by other image?

Thank you!

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