Hello,
I'm looking for a protocol that I can use/adapt for the purpose of scaffolding used in cultivated Meat.
Any tips,
Thanks Jordan
Hi all, Hoping for some troubleshooting/advice. Currently setting up a virus typing assay for routine use. I have the assay working with good sensitivity (regularly amplifies samples that are...
09 June 2024 4,468 3 View
I have a question. I made a comparison between women and men on a series of attitudes concerning romantic relationships. Significant differences were found in a line of T-tests, but I also wanted...
08 May 2024 6,025 7 View
Hello, In the lab we use baytril (enrosite) as an antibacterial post surgery. It comes ready to inject for horses but we need to dilute it for our mice to give them the correct dose. The thing...
30 April 2024 2,844 0 View
currently conducting a study trying to figure out the secondary metabolites from bacteria. Question #1: does it make sense if our flow goes from: extraction > TLC > SEC > TLC >...
23 April 2024 3,602 1 View
Hello, I am using Trypsin to determine its Km and Vmax values against casein from bovine milk. I know higher enzyme concentrations increase the Vmax value, but does more trypsin also affect the...
04 April 2024 8,608 3 View
Hello, Background: I am trying to find the Vmax and Km values for 0.25% trypsin and the substrate casein. During optimization, I have tried different time points (1-15 minutes) and different...
01 April 2024 1,553 1 View
I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...
30 March 2024 8,491 0 View
Hello, I am trying to run a t-test on differences in mental well-being in the last month (average of 14 sentences) between two groups, women I have 200 and men I have only 34 who answered on the...
13 December 2023 8,961 3 View
I have transformed NEB 5-alpha cells with a plasmid that I have pre-sequenced and confirmed it has the correct sequence. All I want to do is make more of this plasmid. However, when I transform...
04 December 2023 8,171 3 View
Hello, i have to transport some primary mouse hepatocytes in some 6 wells plates at 37 degrees celsius ideally. It's going to be a 45 min drive approximately. Does anyone have any ideas or...
06 November 2023 4,039 1 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
Can the kit from Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells on a scaffold?
08 July 2024 7,913 3 View
What did the plantation economy in the South produced and all the crops cultivated in north India cannot cultivated in south India why?
26 June 2024 7,264 0 View
I would like to become self disciplined and work by myself without supervision. How do I achieve it when I am not interested at all in working without any push by others?
24 June 2024 4,529 5 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,608 3 View
"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...
16 June 2024 9,139 1 View
One of the major objectives of Natural Farming is self-reliant farmer and promotion of traditional varieties so that the farmers can use their own seeds. But it is observed that most of the...
11 June 2024 5,696 0 View
For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...
04 June 2024 4,496 0 View
Does dry weather limit tillage options for timing cultivation to complement conservation plans?
02 June 2024 1,734 5 View