What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Can anyone help me troubleshoot QPCR for AAV titering?

So the lab that I work in generates a lot of AAVs and we titer them via QPCR. However, I've been noticing that the entire time, the efficiency has always been very low (anywhere from 40-80%) with...

22 November 2020 4,580 3 View

Can plant tissues be stored at -20C for DNA extraction?

Hey everyone. I collected some leaf tissue samples from the plant Phragmites australis from which I am hoping to extra DNA for sequencing. I will be extracting DNA using Qiagen DNeasy Plant Mini...

08 November 2020 4,989 12 View

Does anyone know of a marker expressed on all lymph-resident cells that can be used in flow cytometry?

I am looking for a marker found on all cells that reside in the lymph node - I study the immune response in salivary glands and we want to gate out any lymph resident cells to ensure that there is...

13 October 2020 6,322 2 View

Can you expect to see changes in secondary structure in a MD simulation?

While running a simulation on any peptide or protein, is it possible for the secondary structures to change? For example, seeing a steady rise in helical content over the whole simulation?

30 September 2020 4,298 9 View

What are ideal personal desktop hardware specs for comparing models for time series analysis and machine learning?

I am researching trading strategies, particularly involving time series analysis, but also machine learning, on data sets that range as far back as 1950 and would like to compare the accuracy of...

15 July 2020 7,733 3 View

How do I determine if my Fst values are significantly different from 0 or between populations that are and are not separated by a highway?

I am trying to determine if a highway is too much of a barrier for shrews to cross and breed freely. What I have to work with is ~1080 bp of the mitochondrial cytochrome b gene (we did not do...

22 June 2020 7,647 1 View

What is the best wearable EEG recording device for research?

Hi there, I am searching a wearable EEG device with as many channels and the highest accuracy possible. I found some papers which reviewed/used the following devices, but still not sure which is...

19 May 2020 5,712 2 View

Help needed with the anatomy of a mouse knee. What tissue is this?

Hi all, I have been collecting cross-sections of a mouse knee joint, and I keep seeing some differently coloured, yellowish tissue on the anterior side of the joint, but I'm not too sure what this...

27 January 2020 5,792 3 View

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02 March 2021 3,060 3 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Can anyone help to find any research articles related to the below mentioned objective?

Astaxanthin used in surimi based products as colour enhancer or as an antioxidant! Regards & Thanks Asik Ikbal

25 January 2021 9,399 4 View

How can I take an electrical conductivity test from a nanofibrous scaffold?

I have a nanofiber scaffold for tissue engineering. When I did the electrical conductivity test, the ohmmeter reports a wide range of values (from 300 kOhm to 20 MOhm). I placed the scaffold...

06 January 2021 2,232 1 View

Extraction of heavy metals in dry meat and fish sold in market along the high ways?

I am doing extraction of heavy metals in dry meat and fish sold in market along the high ways The project requires me to use different extraction method. Can anyone help.

04 January 2021 719 3 View

What is this culture medium for?

Here is the composition of a culture medium named BM medium. It doesn't contain carbon source and nitrogen source, while after cultivation something grow on that. Could anyone explain what...

31 December 2020 8,060 5 View

Does anyone recycle cell culture media and if there is a person what is the procedure and WHY do they do that?

our team researching to cultivated meat, and there is some question related to cell culture media and recycling cell culture media: 1) is cell-cultured media recyclable? 2) has anyone recycled...

27 December 2020 1,467 4 View

Is there any technique to reduce carbonate precentage in bone sample (bovine hydroxyapatite)?

Dear colleagues, I am a researcher working with bovine hydroxyapatite. I want to reduce the percentage of carbonate on the sample. Is there any suggested techniques for this? I am measured the...

19 November 2020 3,828 3 View