There are several steps to prepare the sample using the Nextera XT protocol. At some points we use TCY plate, at other points we use MIDI plate. Also when you seal the plate with the microseal, there is microseal A and B. I am wondering what are the differences? Thanks.
For very low through put I processed samples in 0.2ml PCR tubes and carried out the clean up steps in 1.5ml tubes with reasonable results. For higher through put I used a regular 96 well PCR plate (microseal skirted). The plates in the protocol are very expensive and I was only processing up to 24 samples at a time so there would have been a lot of dead space.
The difference between the Microseal A and Microseal B seals is the optical integrity of the seal. Microseal B adhesive seals are approved for use in Real-Time PCR while Microseal A films are not designed for qPCR purposes. Both are effective seals for transport and use in a thermal cycler but, Microseal B seals should be used for any probe based/quantitative PCR use.
Hope this helps!
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