Hi everyone!
Now we staying at home so I have all the time for any lengthy protocol even manual. the problem is I add my pictures to the IHC toolbox it just takes ages (nothing appearing after half an hour!) and in the end, it is circling the areas that I don't want at all. I have tried other tools and also color deconvolution as recommended by some but plugin itself saying that it cant be used to calculate the intensity.
I exactly want to know how many percentages of the blue nuclei are overlapping with brown in each frame? what is the intensity of each nucleus colored in brown? Unfortunately lots of papers not really showing their pipeline in material methods and now I am pretty confused about which method or plugin works best? IHC gurus please help!