I'm starting a seasonal research project where I'm isolating bacteria from a water source. I'm curious if I collect water right now and isolate bacteria, do I need to set the incubator at the current air temp or can I incubate at higher temps?
I'm conducting protein-protein interaction studies using a LexA-based Bacterial-Two Hybrid system with E. coli reporter strains. We employ MacConkey agar and β-galactosidase assays to evaluate the...
26 May 2024 4,647 0 View
Hi, I have having an issue with the Fc blocking stages when carrying out flow cytometry on a murine macrophage cell line. I am using a rat anti-mouse antibody against the scavenger receptor...
22 April 2024 3,466 2 View
I'm trying to transfect primary PBMCs with siRNA in a Lonza 4-D Nucleofector, but I've had no luck. So far, I've been using Lonza's Amaxa unstimulated T-cell protocols, in the 20 uL/well 2x8-well...
07 August 2023 3,698 0 View
I have tried conducting iba1 and GFAP staining in 40um sliced rat brain sections. The staining seems to work well on the peripheral parts of the brain but not the central. There are the occasional...
11 November 2022 8,447 3 View
Does anyone have any experience of measuring PAHs from urine using GC-MS and any suggestions or published methods that may be helpful? Getting the full method is often very difficult from some papers.
19 January 2021 1,848 3 View
Essentially every published review paper on the MAX phases includes Sc2InC in its listings of 'known MAX phases'. However, most of these reviews either: 1) specifically do not reference Sc2InC,...
04 August 2020 2,223 3 View
Hello, I am interested in determining whether the binding of our antibody to a cell membrane receptor causes the receptor to be internalized or not. We have data that suggests the antibody...
20 January 2020 8,609 6 View
I am looking for a copy of the original Felitti et al (1998) Adverse Experiences Questionnaire? (ACE) I found this copy not sure if it is the correct...
02 January 2020 3,146 0 View
I have recently started doing western blots in my lab. After transferring my gel onto the nitrocellulose membrane. I wash the blot 1 time with TBST then block with 5% non-fat milk for 1 hour at...
31 October 2019 4,820 2 View
Initially I was going to use a binary logistic regression. I cannot find a good reason to make the beneish M-score into a binary variable. I did this because I saw this widely used in...
31 May 2019 6,639 7 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View