How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Should absorbance be between 0 and 1 for ELISA?

Hello, I am running a sandwich-type ELISA for which the readout is absorbance at 450nm. The ELISA measures protein binding which I am screening for compounds to inhibit. The 570nm...

05 October 2020 9,813 6 View

Why do we use HHL as a substrate in studying ACE enzyme inhibition activity assay?

Need to know from the community why we use Hippury-L-histidyl-L-Leucine as a substrate in ACE inhibitory activity

20 September 2020 1,040 5 View

Are Angiotensin Receptor Blocker Trials as Therapy Inhibiting COVID 19 Infection Successful?

There has been some discussion about the comorbidities, including cardiovascular disease and diabetes, which often qualify for angiotensin inhibitor (ACEI) therapy, which significantly increase...

27 April 2020 172 36 View

Is ACE inhibition linked to COVID-19?

There is increasing chatter that proteins in the ACE2 receptor binding domain are related to COVID-19 entering humans cells and subsequently infecting the host. Is there any evidence of ACE...

19 March 2020 6,635 8 View

ACE Inhibition Assay?

1) I am curious on what solution should I used to dilute the ACE enzyme to respected concentration? is it the same regardless of what solution? or the activity will be differ accordingly to the...

24 February 2020 9,530 2 View

Could areas of high Fluoride ingestion be more susceptible to Coronavirus outbreaks?

The US Government Comparative Toxicogenomics database shows that Fluoride can inhibit Human immunity to viruses and pneumonia. Angiotensin I-Converting Enzyme (ACE), 2'-5'-Oligoadenylate...

03 February 2020 6,459 4 View

ACE Inhibition Assay?

1) I am curious on what solution should I used to dilute the ACE enzyme to respected concentration? is it the same regardless of what solution? or the activity will be differ accordingly to the...

01 February 2020 2,135 1 View

How to resolve FLIR bad quality output issue?

Hello, I have a FLIR VUE PRO R 640 Thermal Camera fixed mounted on my custom Drone. For the last couple of months, this sensor is not capturing the quality data and the use of FLIR TOOLS with...

26 November 2019 4,718 3 View

How to resolve FLIR bad quality output issue?

Hello, I have a FLIR VUE PRO R 640 Thermal Camera fixed mounted on my custom Drone. For the last couple of months, this sensor is not capturing the quality data and the use of FLIR TOOLS with...

10 November 2019 1,690 3 View