Can anyone provide some literature (reviews)pertaining to murine macrophages & human monocytes?

Hiii can anyone please provide some good literature especially reviews or book chapter on murine macrophages & human monocytic cells which can be downloaded for free

08 September 2017 6,251 2 View

How to estimate the total number of nanoparticles present in an solution?

I had prepared my nanoparticles by ultrasonic degradation of the polysaccharide. DLS studies showed that the PdI was 0.2. Now how can I conclude that what is the number of nanoparticles present in...

08 September 2017 6,482 10 View

Coupling of proteins with polysaccharide derived nanoparticles?

08 September 2017 7,134 6 View

How to proceed for XRD of nanoparticles dispersed in non-volatile solvents like water?

I have my polysaccharide derived nanoparticles dispersed in water. I am interested in doing XRD analysis of my sample but haven't done this analysis before. Can anybody give me an insight how can...

04 May 2017 1,199 5 View

Concentration of CBA flex set capture beads and IFN-gamma antibodies to be used?

I am trying to detect the IFN-gamma levels (quantitative analysis) in J774 cells treated with specific polysaccharides using CBA Flex set from BD Biosciences. the kit does not tells about the...

01 February 2017 6,714 8 View

How to make nanoparticles less than 10nm form Beta-glucan polymers?

the nanoparticles need to be internalized into cell line J774.

05 June 2016 7,064 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View